Enhanced Therapeutic Efficacy of iRGD-Conjugated Crosslinked Multilayer Liposomes for Drug Delivery
Figure 2
In vitro cytotoxicity, binding, and internalization of iRGD-cMLVs and cMLVs in tumor cells. ((a), (b)) In vitro cytotoxicity of cMLV(Dox) and iRGD-cMLV(Dox) in 4T1 tumor (a) and multidrug-resistant JC cells (b). The cytotoxicity was measured by a standard XTT assay. Error bars represent the standard deviation of the mean from triplicate experiments. ((c), (d)) Binding and internalization of cMLV(Dox) and iRGD-cMLV(Dox) to 4T1 cells. 4T1 cells were incubated with cMLV(Dox) and iRGD-cMLV(Dox) for 30 min at 4°C (c) or 2 h at 37°C (d). Both binding and cellular uptake of nanoparticles were determined by measuring doxorubicin fluorescence using flow cytometry. Statistical analysis was performed with Student’s t-test. Error bars represent the standard deviation of the mean from triplicate experiments.
