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BioMed Research International
Volume 2013 (2013), Article ID 382063, 7 pages
http://dx.doi.org/10.1155/2013/382063
Research Article

Enzymes Inhibition and Antidiabetic Effect of Isolated Constituents from Dillenia indica

1Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra, Haryana 136119, India
2Department of Pharmacy, School of Chemical Sciences and Pharmacy, Central University of Rajasthan, Ajmer 305801, India

Received 11 April 2013; Revised 24 September 2013; Accepted 25 September 2013

Academic Editor: Ronald E. Baynes

Copyright © 2013 Sunil Kumar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aims. This study was designed to investigate the enzyme inhibitory and antidiabetic activity for the constituents isolated from Dillenia indica. Methods. The leaves of D. indica were extracted with methanol and subjected to fractionation and chromatographic separation, which led to the isolation of seven compounds: betulinic acid (1), n-heptacosan-7-one (2), n-nonatriacontan-18-one (3), quercetin (4), β sitosterol (5), stigmasterol (6), and stigmasteryl palmitate (7). Among these isolates, compounds 1, 4, 5, and 6 were evaluated for in vitro enzyme inhibition and compounds 4, 5 and 6 were evaluated for antidiabetic activity in streptozotocin-nicotinamide induced diabetic mice. Results. Compounds 1, 4, 5, and 6 showed 47.4, 55.2, 48.8, and 44.3% α-amylase inhibition, respectively, and 52.2, 78.2, 52.5, and 34.2% α-glucosidase inhibition, respectively, at the dose of 50 µg/kg. Compounds 4, 5 and 6 also showed significant (* ) antidiabetic activity in streptozotocin-nicotinamide induced diabetic mice at the dose of 10 mg/kg. Conclusion. These results provide evidence that Dillenia indica might be a potential source of antidiabetic agents.