Salamander-Derived, Human-Optimized nAG Protein Suppresses Collagen Synthesis and Increases Collagen Degradation in Primary Human Fibroblasts
Figure 6
Quantitative real-time PCR (RT-PCR) measuring relative mRNA expressions level of procollagen I and procollagen III in non-transfected and nAG transfected primary human fibroblasts. 100 ng of total RNA was reverse-transcribed and target genes expression was measured in multiplex, one-step RT-PCR by using TaqMan probes with (FAM, HEX, or ROX) reporter dyes and (BHQ1 or BHQ2) quencher. To estimate effect of nAG on collagen I and collagen III synthesis, the relative mRNA expressions were related to the reference gene, β-actin. The relative expression of procollagen I was 55% decrease () and procollagen III was 95% decrease () in nAG transfected fibroblasts compared to non-transfected fibroblasts. The data represents the mean of three independent experiments.
