Detection of EGFR Mutations by TaqMan Mutation Detection Assays Powered by Competitive Allele-Specific TaqMan PCR Technology

Table 2

EGFR mutation detection in NSCLC samples.

Training set						Validation set
Sample	Sample type	Tumor cells	Standard methods	CastPCR	Mutation	Sample	Sample type	Tumor cells	Standard methods	CastPCR	Mutation
731	FFPE (S)*	70%	∘	∘	WT	2826	FFPE (S)	80%	∘	∘	WT
732	FFPE (S)	50%	∘	∘	WT	2832	FFPE (S)	30%	∘	∘	WT
828	FFPE (S)	70%	•	•	Del15bp_ex19	2843	Cytology	300 cells	•	•	p.G719A
913a	FFPE (B)**	30%	•	•	p.L858R	2858	FFPE (S)	40%	•	•	p.L858R
966	FFPE (S)	70%	•	•	p.L858R	2903	FFPE (S)	30%	∘	∘	WT
1070b	FFPE (S)	90%	∘	∘	WT	2953	Cytology	1000 cells	•	•	Del9bp_ex19
1232a	FFPE (B)	60%	•	•	Del15bp_ex19	2958	FFPE (S)	90%	•	•	p.L858R-p.T790M
1262	FFPE (S)	40%	•	•	Del15bp_ex19	2965	FFPE (S)	40%	∘	∘	WT
1406	Cytology	1500 cells	∘	∘	WT	2971	Cytology	5000 cells	∘	∘	WT
1591	Cytology	>500 cells	•	•	p.G719S	2988	FFPE (S)	70%	∘	∘	WT
1674	FFPE (S)	50%	•	•	Del18bp_ex19	2992	FFPE (S)	80%	•	•	Del9bp_ex19
1677	FFPE (S)	60%	•	•	L858R	3023	FFPE (S)	60%	∘	∘	WT
2139	FFPE (S)	80%	∘	∘	WT	3031	FFPE (B)	20%	∘	∘	WT
2355	FFPE (S)	90%	∘	∘	WT	3032	FFPE (S)	20%	•	•	p.L861Q
2376	FFPE (B)	60%	•	•	Del12bp_ex19	3053	FFPE (B)	50%	∘	∘	WT
2572	Cytology	300 cells	•	•	Del15bp_ex19	3060	FFPE (S)	10%	∘	∘	WT
2659	Cytology	5000 cells	∘	∘	WT	3070	Cytology	150 cells	∘	∘	WT
2665	Cytology	250 cells	∘	∘	WT	3089	FFPE (B)	80%	∘	∘	WT
2693	Cytology	50%	∘	∘	WT	3098	FFPE (S)	70%	∘	∘	WT
2722	FFPE (S)	70%	•	•	Del15bp_ex19	3111	FFPE (B)	20%	∘	∘	WT
2733	Cytology	100 cells	∘	∘	WT	3140	FFPE (S)	20%	•	•	Del18bp_ex19
2739	FFPE (B)	40%	∘	∘	WT	3171	FFPE (S)	60%	•	•	p.L858R
2762	FFPE (B)	30%	•	•	Del15bp_ex19	3000a	FFPE (B)	5%	•	∘	Discordant p.L858R
2767	FFPE (B)	40%	•	•	p.L858R	2768	FFPE (B)	50%	∘	•	Discordant p.L858R
2778	Cytology	500 cells	∘	∘	WT	1672	Cytology	500 cells	•	∘	Discordant Del18bp_ex19
2787	FFPE (S)	80%	∘	∘	WT	2527	FFPE (S)	70%	•	∘	Discordant Del24bp_ex19
2789	FFPE (S)	80%	•	•	p.L858R	3014	FFPE (S)	80%	•	∘	Discordant ins9bp_ex20
2790	Cytology	250 cells	∘	∘	WT	3045	FFPE (S)	70%	•	∘	Discordant ins3bp_ex20
2798	FFPE (S)	90%	∘	∘	WT
2824	FFPE (S)	80%	∘	∘	WT

∗FFPE tissue, surgical specimen; ∗∗FFPE tissue, small biopsy.