Research Article
Lignocellulosic Fermentation of Wild Grass Employing Recombinant Hydrolytic Enzymes and Fermentative Microbes with Effective Bioethanol Recovery
Table 3
Different SSF combinations of recombinant hydrolytic enzymes and fermentative microbes with steam exploded wild grass.
| | SSF combination | Substrate concentration (%, w/v) and mode of SSF | Reducing sugar* (g·L−1) | Ethanol yield (g of ethanol·g of substrate−1) | Ethanol titre* (g·L−1) |
| | GH5 + S. cerevisiae | 1% shake flask | 1.26 ± 0.06 | 0.067 | 0.67 ± 0.02 | | GH5 + GH43 + S. cerevisiae + C. shehatae (shake flask) | 1% shake flask | 1.60 ± 0.05 | 0.128 | 1.28 ± 0.03 | | GH5 + GH43 + S. cerevisiae + C. shehatae (shake flask) | 5% shake flask | 13.0 ± 0.04 | 0.224 | 11.20 ± 0.06 | | GH5 + GH43 + S. cerevisiae + C. shehatae (bioreactor) | 5% bioreactor | 30.0 ± 0.04 | 0.461 | 23.07 ± 0.08 |
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The values correspond to the maximum reducing sugar and maximum ethanol at a particular time; values are mean ± SE ().
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