A Review of Haptoglobin Typing Methods for Disease Association Study and Preventing Anaphylactic Transfusion Reaction
Figure 3
A schematic representation of conventional PCR results for Hp genotyping. (a) Gel electrophoresis to distinguish the allele from other alleles [13]. A band of 315 bp size is specific for the allele, and a larger band (476 bp) represents the presence of alleles other than ( or alleles). (b) A representative image of PCR products to determine the and genotypes [14, 15]. The amplification of the allele results in a large-sized amplicon (3481 or 4370 bp according to the primer design), and the allele is amplified to produce a relatively smaller band (1757 or 3000 bp). The larger band (>3.0 kb) is very weak and sometimes not detected, especially in the presence of a smaller band (see text for details).
