Identification, Selection, and Enrichment of Cardiomyocyte Precursors
Figure 4
Characterization of CMPs by immunocytochemistry and RT-PCR. (a) ADSCs transduced with lentivectors before G418 selection (first column). These cells were selected with G418, expanded, and stained with anti-desmin (second column), anticardiac troponin T (third column), antismooth muscle actin (fourth column), and anti-von Willebrand factor (fifth column) antibodies. The nontransduced ADSCs were used as control (first line) and ECV304 cells were used as positive control for smooth muscle actin and von Willebrand factor staining. (b) RT-PCR of the selected cells for the cardiomyocyte-specific genes. The GAPDH gene was used as control. The RNAs extracted from a human heart and ECV were used as positive and negative controls, respectively.
