Evaluation of hsp65 Nested PCR-Restriction Analysis (PRA) for Diagnosing Tuberculosis in a High Burden Country

Table 1

Detection of Mycobacterium spp by hsp65 Nested PCR-PRA, culture, and AFB smear.

	hsp65 Nested PRC-PRA
	Positive (%)	Negative (%)	Total (%)	𝑃 value
Culture				<0.0000001
Positive	27 (21.25; 95% CI 15.04–29.16)	0 (0.0; 95% CI 0.0–2.94)	27 (21.25; 95% CI 15.04–29.16)
Negative	5 (3.94; 95% CI 1.69–8.89)	95 (74.80; 95% CI 66.6–81.55)	100 (78.74; 95% CI 70.84–84.96)
Total	32 (25.20; 95%  CI 18.45–33.4)	95 (74.80; 95%  CI 66.6–81.55)	127 (100.0; 95%  CI 97.06–100)
AFB* smear				<0.0000001
Positive	26 (20.47; 95% CI 14.37–28.31)	0 (0.0; 95% CI 0.0–2.94)	26 (20.47; 95% CI 14.37–28.31)
1+	11 (8.66; 95% CI 4.91–14.84)
2+	7 (5.51; 95% CI 2.70–10.94)
3+	8 (6.30; 95% CI 3.23–11.94)
Negative	7 (5.51; 95% CI 2.70–10.94)	94 (74.02; 95% CI 65.76–80.86)	101 (79.52; 95% CI 71.69–85.63)
Total	33 (25.98; 95%  CI 19.14–34.24)	94 (74.02; 95%  CI 65.76–80.86)	127 (100.0; 95%  CI 97.06–100)

∗AFB: acid fast bacilli.