Research Article
Evaluation of hsp65 Nested PCR-Restriction Analysis (PRA) for Diagnosing Tuberculosis in a High Burden Country
Table 1
Detection of Mycobacterium spp by hsp65 Nested PCR-PRA, culture, and AFB smear.
| | hsp65 Nested PRC-PRA | | Positive (%) | Negative (%) | Total (%) | value |
| Culture | | | | 0.0000001 | Positive | 27 (21.25; 95% CI 15.04–29.16) | 0 (0.0; 95% CI 0.0–2.94) | 27 (21.25; 95% CI 15.04–29.16) | Negative | 5 (3.94; 95% CI 1.69–8.89) | 95 (74.80; 95% CI 66.6–81.55) | 100 (78.74; 95% CI 70.84–84.96) | Total | 32 (25.20; 95% CI 18.45–33.4) | 95 (74.80; 95% CI 66.6–81.55) | 127 (100.0; 95% CI 97.06–100) | AFB* smear | | | | 0.0000001 | Positive | 26 (20.47; 95% CI 14.37–28.31) | 0 (0.0; 95% CI 0.0–2.94) | 26 (20.47; 95% CI 14.37–28.31) | 1+ | 11 (8.66; 95% CI 4.91–14.84) | | | 2+ | 7 (5.51; 95% CI 2.70–10.94) | | | 3+ | 8 (6.30; 95% CI 3.23–11.94) | | | Negative | 7 (5.51; 95% CI 2.70–10.94) | 94 (74.02; 95% CI 65.76–80.86) | 101 (79.52; 95% CI 71.69–85.63) | Total | 33 (25.98; 95% CI 19.14–34.24) | 94 (74.02; 95% CI 65.76–80.86) | 127 (100.0; 95% CI 97.06–100) |
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AFB: acid fast bacilli.
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