Table 1: Detection of Mycobacterium spp by hsp65 Nested PCR-PRA, culture, and AFB smear.

hsp65 Nested PRC-PRA
Positive (%)Negative (%)Total (%) value

Culture 0.0000001
 Positive27 (21.25; 95% CI 15.04–29.16)0 (0.0; 95% CI 0.0–2.94)27 (21.25; 95% CI 15.04–29.16)
 Negative5 (3.94; 95% CI 1.69–8.89)95 (74.80; 95% CI 66.6–81.55)100 (78.74; 95% CI 70.84–84.96)
 Total32 (25.20; 95%  CI 18.45–33.4)95 (74.80; 95%  CI 66.6–81.55)127 (100.0; 95%  CI 97.06–100)
AFB* smear 0.0000001
 Positive26 (20.47; 95% CI 14.37–28.31)0 (0.0; 95% CI 0.0–2.94)26 (20.47; 95% CI 14.37–28.31)
  1+11 (8.66; 95% CI 4.91–14.84)
  2+7 (5.51; 95% CI 2.70–10.94)
  3+8 (6.30; 95% CI 3.23–11.94)
 Negative7 (5.51; 95% CI 2.70–10.94)94 (74.02; 95% CI 65.76–80.86)101 (79.52; 95% CI 71.69–85.63)
 Total33 (25.98; 95%  CI 19.14–34.24)94 (74.02; 95%  CI 65.76–80.86)127 (100.0; 95%  CI 97.06–100)

AFB: acid fast bacilli.