Lovastatin-Enriched Rice Straw Enhances Biomass Quality and Suppresses Ruminal Methanogenesis

Table 1

Names, sequences, application, product size, annealing temperature and references of the primers used.

Target group	Sequence 5′–3′	Application	Product size (bp)	Annealing temperature (°C)	Reference
Total bacteria F	CGGCAACGAGCGCAACCC	Quantification	145	55	[12]
Total bacteria R	CCATTGTAGCACGTGTGTAGCC
Total fungi F	GAGGAAGTAAAAGTCGTAACAAGGTTTC	Quantification	121	55	[13]
Total fungi R	CAAATTCACAAAGGGTAGGATGATT
Methanobacteriales F (MBT857F)	CGWAGGGAAGCTGTTAAGT	Quantification	343	58	[14]
Methanobacteriales R (MBT1196R)	TACCGTCGTCCACTCCTT
Ruminococcus albus F	CCCTAA AAGCAGTCTTAGTTCG	Quantification	175	55	[12]
Ruminococcus albus R	CCTCCTTGCGGTTAGAACA
Ruminococcus flavefaciens F (Rf154f)	TCTGGAAACGGATGGTA	Quantification	259	60	[12]
Ruminococcus flavefaciens R (Rf425r)	CCTTTAAGACAGGAGTTTACAA
Fibrobacter succinogenes F	GTTCGGAATTACTGGGCGTAAA	Quantification	122	55	[13]
Fibrobacter succinogenes R	CGCCTGCCCCTGAACTATC
Protozoa F	CTTGCCCTCYAATCGTWCT	Quantification	223	55	[15]
Protozoa R	GCTTTCGWTGGTAGTGTATT
Methanogens (mcrA)-F	TTCGGTGGATCDCARAGRGC	Quantification gene expression	140	58	[16]
Methanogens (mcrA)-R	GBARGTCGWAWCCGTAGAATCC
HMG-CoA reductase R	GGCTGTGAATTACCGCATATGG	Gene expression	117	55	[17]
HMG-CoA reductase F	TAACGGTCCGGCTACACCTACA
16S rRNA F	CCGGAGATGGAACCTGAGAC	Gene expression (reference gene)	160	55	[18]
16S rRNA R	CGGTCTTGCCCAGCTCTTATTC