Promotion of Hepatic Differentiation of Bone Marrow Mesenchymal Stem Cells on Decellularized Cell-Deposited Extracellular Matrix

Figure 4

Cell culture of BMSCs on TCPS and cell-deposited ECM. Morphological changes and density of BMSCs were detected by FDA staining (a). ECM improved proliferation of BMSCs in 5-day culture (b). BMSCs on ECM showed a lower level of ROS than the cells on TCPS (c). mRNA levels of CuZn-SOD (d) and Mn-SOD (e) in the cultured cells were determined by real-time RT-PCR. Scale bar = 100 μm. All values are mean ± S.E. of independent 4∼5 experiments performed (proliferation assay  𝑛=5; ROS assay  𝑛=4; PCR  𝑛=4); *𝑃 < 0.05.

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