Androgen Signaling Disruption during Fetal and Postnatal Development Affects Androgen Receptor and Connexin 43 Expression and Distribution in Adult Boar Prostate
Figure 4
Proliferating nuclear antigen (PCNA) and caspase 3 protein expression in prostates of control and flutamide-exposed boars. (a) Immunofluorescent detection of PCNA in control prostate section (white arrows). Nuclei were counterstained with DAPI (blue). Scale bar represents 20 μm ((b), (c)). Representative immunoblots for PCNA (b) and caspase 3 (c). The band at 32 kDa represents inactive proenzyme (procaspase 3) and the band at 17 kDa corresponds to active form (cleaved caspase 3). Actin was used as a loading control, and each set of shown actin immunoblots corresponds to the target protein that was investigated within a given panel. The relative level of PCNA (b) and cleaved caspase 3 (c) protein normalized against its corresponding -actin. Data obtained from three separate analyses is expressed as mean ± SD. Significant differences from control values are denoted as *, **, and ***.
