Fractionation and Purification of Bioactive Compounds Obtained from a Brewery Waste Stream

Table 3

Phenolic compounds present in the different fractions, obtained with a LC-18 column, identified and quantified by HPLC-DAD.

	Fraction											Crude extract
Antioxidant compound	1	2	3	4	5	6	7	8	9	10	11	—
	mg g^−1 of fraction											mg g^−1
Gallic acid	193	—	—	—	—	—	—	—	—	—	—	20.1
Gallocatechin	178	641	—	—	—	—	—	—	—	—	—	132
Protocatechuic acid	—	72.9	9.51	—	—	—	—	—	—	—	—	15.5
Epigallocatechin		86.2	96.4									30.6
Catechin	—	114	75.5	—	—	—	—	—	—	—	—	29.9
4-Hydroxybenzoic acid	—	6.51	7.49	—	—	—	—	—	—	—	—	2.54
Caffeic acid	—	13.7	66.0	—	—	—	—	—	—	—	—	14.1
Epicatechin	—	—	132	—	—	—	—	—	—	—	—	21.4
p-coumaric acid	—	—	73.9	1.83	—	—	—	—	—	—	—	11.4
Isoquercetin	—	—	166	—	—	—	—	—	—	—	—	28.3
Ferulic acid	—	—	29.6	138	—	—	—	—	—	—	—	33.7
Acetosyringone	—	—	35.2	50.9	—	—	—	—	—	—	—	14.6
Resveratrol	—	—	—	—	—	51.6	14.5	—	—	—	—	5.35
Quercetin	—	—	—	—	31.7	117	—	—	—	—	—	14.5
Apigenin	—	—	—	—	—	58.8	59.7	—	—	—	—	8.10
Kaempferol	—	—	—	—	—	25.3	23.4	120	—	—	—	6.06
Naringenin	—	—	—	—	—	38.7	171	11.8	—	—	—	14.6
Total (mg g^−1)	371	934	692	191	31.7	292	269	132	—	—	—	403