Fractionation and Purification of Bioactive Compounds Obtained from a Brewery Waste Stream

Table 6

Phenolic profile of each fraction obtained under SFE conditions of test E: temperature 40°C, extraction time 30 minutes, modifier ethanol (6%), and modifier flow 0.2 mL min⁻¹. Phenolic compounds are expressed as mg g⁻¹ of fraction.

Peak no.	Phenolic compound	SFE fractions (mg g^−1)
		ECell	E100	E120	E140	E160	E200	E250	E300
1	Gallic acid	1.04	0.24	1.05	1.68	2.61	3.02	3.44	3.07
2	Gallocatechin	171	10.7	14.8	33.9	41.0	33.3	29.8	20.9
3	Protocatechuic acid	—	1.99	4.12	9.19	12.8	14.9	16.7	16.0
4	Epigallocatechin	1.43	10.7	10.2	—	—	—	—	—
5	Catechin	6.10	3.40	3.36	6.88	11.8	11.7	15.7	16.7
6	4-Hydroxybeinzoic acid	—	3.58	1.96	4.78	4.22	2.72	2.36	1.55
7	Caffeic acid	—	7.29	7.38	17.1	19.65	14.8	13.3	9.52
8	Epicatechin	—	—	—	—	0.23	0.14	0.17	0.40
9	p-coumaric acid	—	7.97	5.89	13.5	13.9	9.66	8.45	6.26
10	Isoquercetin	2.51	—	—	—	—	3.71	3.75	1.43
11	Ferulic acid	—	35.7	27.6	69.0	70.1	47.0	38.9	22.0
12	Acetosyringone	—	2.92	0.83	3.62	2.07	1.18	3.10	2.55
13	Resveratrol	—	—	0.54	1.68	0.37	0.46	0.60	0.80
14	Quercetin	0.57	0.65	0.98	1.91	2.61	2.83	3.13	2.96
15	Apigenin	—	—	0.04	0.66	0.78	0.72	0.79	0.93
16	Kaempferol	1.48	0.94	1.65	3.17	4.42	3.18	6.24	6.64
17	Naringenin	0.39	0.40	0.78	1.69	2.45	2.75	3.26	3.52
	Total (mg g^−1)	11.0	88.9	82.5	172.7	192.8	155.9	152.3	117.0