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BioMed Research International
Volume 2013 (2013), Article ID 410631, 8 pages
http://dx.doi.org/10.1155/2013/410631
Research Article

A Novel Closed-Chest Porcine Model of Chronic Ischemic Heart Failure Suitable for Experimental Research in Cardiovascular Disease

1Department of Medico-Surgical Sciences and Biotechnologies, Sapienza University of Rome, Corso della Repubblica 79, 04100 Latina, Italy
2Department of Cardiovascular, Respiratory, Nephrologic, Anesthesiologic and Geriatric Sciences, Sapienza University of Rome, Viale del Policlinico 155, 00161 Rome, Italy
3Center of Experimental Research, Catholic University of Rome, Largo Agostino Gemelli 8, 00168 Rome, Italy
4Division of Cardiology, Catholic University of Rome, Largo Agostino Gemelli 8, 00168 Rome, Italy
5Department of Cardiac Surgery, Bambino Gesù Hospital, Piazza di Sant’Onofrio 4, 00165 Rome, Italy
6Division of Cardiology, University of Turin, Corso Bramante 88-90, 10126 Turin, Italy
7IRCCS Neuromed, Via Atinense 18, 86077 Pozzilli, Italy

Received 18 July 2013; Accepted 12 August 2013

Academic Editor: Andrea Vecchione

Copyright © 2013 Giuseppe Biondi-Zoccai et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Cardiac pathologies are among the leading causes of mortality and morbidity in industrialized countries, with myocardial infarction (MI) representing one of the major conditions leading to heart failure (HF). Hitherto, the development of consistent, stable, and reproducible models of closed-chest MI in large animals, meeting the clinical realism of a patient with HF subsequent to chronic ischemic necrosis, has not been successful. We hereby report the design and ensuing application of a novel porcine experimental model of closed-chest chronic ischemia suitable for biomedical research, mimicking post-MI HF. We also emphasize the key procedural steps involved in replicating this unprecedented model, from femoral artery and vein catheterization to MI induction by permanent occlusion of the left anterior descending coronary artery through superselective deployment of platinum-nylon coils, as well as endomyocardial biopsy sampling for histologic analysis and cell harvesting. Our model could indeed represent a valuable contribution and tool for translational research, providing precious insights to understand and overcome the many hurdles concerning, and currently quenching, the preclinical steps mandatory for the clinical translation of new cardiovascular technologies for personalized HF treatments.