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BioMed Research International
Volume 2013 (2013), Article ID 410686, 9 pages
http://dx.doi.org/10.1155/2013/410686
Research Article

Enrichment, Development, and Assessment of Indian Basil Oil Based Antiseptic Cream Formulation Utilizing Hydrophilic-Lipophilic Balance Approach

1Herbal Medicinal Products Department, CSIR-Central Institute of Medicinal and Aromatic Plants, PO-CIMAP, Lucknow 226015, India
2Molecular Bioprospection Department, CSIR-Central Institute of Medicinal and Aromatic Plants, PO-CIMAP, Lucknow 226015, India

Received 12 April 2013; Accepted 9 July 2013

Academic Editor: Ismail Kiran

Copyright © 2013 Narayan Prasad Yadav et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

In the supporting information some literature and our findings (that are not included in the manuscript) are provided for better understanding and recognition of the applied methodologies. As already discussed in the manuscript, we determined the required hydrophilic-lipophilic balance of basil oil, an important physicochemical property. Implementing the determined rHLB value, we further calculated the rHLB of cream formulation and finally developed a stable cream. The formulations were evaluated for antimicrobial activity and skin irritation index. The supporting information includes some representative photographs of antimicrobial evaluation of cream formulations showing zone of growth inhibition (ZGI) against different microorganisms. Details of skin irritation studies are provided which include experimental protocol, scoring criteria, data and calculation of Primary irritation Index.

  1. Supplementary Material