A Pentaplex PCR Assay for the Detection and Differentiation of Shigella Species
Figure 3
Analytical sensitivity of multiplex PCR at the bacterial level (CFU/mL) using reference strains. Lane 1, 100 bp plus marker; lane 2, positive control; lane 3, 108 CFU/mL lysate of S. flexneri; lane 4, 107 CFU/mL lysate of S. flexneri; lane 5, 106 CFU/mL lysate of S. flexneri; lane 6, 105 CFU/mL lysate of S. flexneri; lane 7, 104 CFU/mL lysate of S. flexneri; lane 8, 103 CFU/mL lysate of S. flexneri; lane 9, 102 CFU/mL lysate of S. flexneri; lane 10, 100 bp plus Marker; lane 11, 108 CFU/mL lysate of S. sonnei; lane 12, 107 CFU/mL lysate of S. sonnei; lane 13, 106 CFU/mL lysate of S. sonnei; lane 14, 105 CFU/mL lysate of S. sonnei; lane 15, 104 CFU/mL lysate of S. sonnei; lane 16, 103 CFU/mL lysate of S. sonnei; lane 17, 102 CFU/mL lysate of S. sonnei; lane 18, 100 bp plus Marker; lane 19, Positive control; lane 20, 108 CFU/mL lysate of S. dysenteriae; lane 21, 107 CFU/mL lysate of S. dysenteriae; lane 22, 106 CFU/mL lysate of S. dysenteriae; lane 23, 105 CFU/mL lysate of S. dysenteriae; lane 24, 104 CFU/mL lysate of S. dysenteriae; lane 25, 103 CFU/mL lysate of S. dysenteriae; lane 26, 102 CFU/mL lysate of S. dysenteriae; lane 27, 100 bp plus marker.
