413793.fig.003
Figure 3: Electrophoretic patterns of plasmid DNA of pUC19 in the presence of non-A. awamori-fermented LPP and A. awamori-fermented LPPs after 3 or 6 days. A, pUC19; B, C, D, E, F, pUC19, damage solution, and non-A. awamori-fermented LPPs at 1, 0.5, 0.2, 0.1, and 0.05 mg/mL; G, pUC19 and damage solution (blank); H, I, J, K, and L, pUC19, damage solution and A. awamori-fermented LPPs after 3 days at 1, 0.5, 0.2, 0.1, and 0.05 mg/mL; M, N, O, P, and Q, pUC19, damage solution and A. awamori-fermented LPP after 6 days at 1, 0.5, 0.2, 0.1, and 0.05 mg/mL. The damage solution containing 50 mM OH generated by mixing 2 μL of 50 mM hydrogen peroxide and 2 μL of 5 mM ferrous sulfate. Supercircular DNA was indicated as S form, linear DNA is indicated as L form, and open circular DNA is indicated as O form.