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Figure 5: Effect of saffron on exponentially growing A549 cells. Scattergrams of LSC measurements representing the cells stained with FAM-VAD-FMK (green fluorescence) and PI (red fluorescence). (A) Control cells exponentially growing in standard condition. (B) Cells exposed to 200 μg/mL saffron for 48 h during exponential growth. (C) Cells exposed to 400 μg/mL saffron for 48 h during exponential growth. Each scatterplot is divided into four compartments depending on the cell status as revealed by green versus red fluorescence. a: live cells; b: early apoptotic cells; c: late apoptotic cells; d: secondary necrotic cells.