Expression Pattern of Genes of RLR-Mediated Antiviral Pathway in Different-Breed Chicken Response to Marek’s Disease Virus Infection

Table 1

Genes and primer pairs used in this study.

Genes		Primer pairs sequences (5′–3′)	Annealing temperatures (°C)	Amplicons (bp)	Accession numbers
Meq	Forward; Reverse	ACGCAGGGAGCAGACGTACTAT; CCATAGGGCAAACTGGCTCAT	63°C	155	YP_001033993
MDA-5	Forward; Reverse	GTTGCTGTAGGAGATGCAAGTG; ATCTGGCTCAGGTGAAGCTCT	60°C	114	NM_001193638
IRF-3	Forward; Reverse	TACACTGAGGACTTGCTGGAGGT; AAGATGGTGGTCTCCTGATCC	62°C	170	NM_205372
IFN-α	Forward; Reverse	CAGGATGCCACCTTCTCTCAC; AGGATGGTGTCGTTGAAGGAG	60°C	113	NM_205427
IFN-β	Forward; Reverse	CCTCAACCAGATCCAGCATTAC; CCCAGGTACAAGCACTGTAGTT	59°C	167	NM_001024836
GAPDH	Forward; Reverse	AGGACCAGGTTGTCTCCTGT; CCATCAAGTCCACAACACGG	62°C	153	NM_204305