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BioMed Research International
Volume 2013 (2013), Article ID 426034, 11 pages
http://dx.doi.org/10.1155/2013/426034
Research Article

Full-Length Genomic Sequence of Subgenotype IIIA Hepatitis A Virus Isolate in Republic of Korea

1Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea
2Korea Zoonosis Research Institute, Chonbuk National University, Jeonju 561-756, Republic of Korea
3Environmental Infrastructure Research Department, National Institute of Environmental Research, Incheon 404-708, Republic of Korea

Received 28 February 2013; Revised 28 May 2013; Accepted 31 May 2013

Academic Editor: Fumio Imazeki

Copyright © 2013 Ah-Ra Lee et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Hepatitis A virus is known to cause acute hepatitis and has significant implications for public health throughout the world. In the Republic of Korea, the number of patients with hepatitis A virus infection has been increasing rapidly since 2006. In this study, the Kor-HAV-F strain was identified as subgenotype IIIA by RT-PCR, and its identity was confirmed by nucleotide sequencing and alignment analysis. Moreover, detailed phylogenetic analysis indicated that the Kor-HAV-F strain clustered into subgenotype IIIA, including strains isolated in Japan, Norway, and India. The entire amino acid sequence of the VP1 and 2A regions was compared with that of the reference strains isolated in various countries. We found 2 amino acid changes (T168A and L96P, resp.) in the VP1 and 2A regions, which had not been found in any other hepatitis A virus strain. To our knowledge, this study is the first to report the full-length sequence of a hepatitis A virus isolated in the Republic of Korea.