430861.fig.004a
(a)
430861.fig.004b
(b)
Figure 4: Jurkat T cells were exposed to IL-2 (250 U/mL) after using 25 μM PD98059 or depletion of ERK with small interfering RNA (siRNA). (a) after using 25 μM PD98059, p-ERK and ERK levels were measured by western blot. CD25 expression was measured by flow cytometry. (b) after depletion of ERK with small interfering RNA (siRNA), p-ERK and ERK levels were measured by western blot. CD25 expression was measured by flow cytometry. Mean ± S.E. ( ). *, .