Clinical Study

Role of Rheum Polysaccharide in the Cytokines Produced by Peripheral Blood Monocytes in TLR4 Mediated HLA-B27 Associated AAU

Figure 1

TNF-α (a) and IL-10 (b) levels in a time-dependent manner. Peripheral blood monocytes (1 × 106 cells/mL) were pretreated for indicated time with 5 mg·L−1 HTA-125 or 100 mg·L-1 RP and then incubated with 1 mg·L−1 LPS for another 24 h. Cell-free supernatants were collected. The relative concentrations of TNF-α and IL-10 in the supernatants were determined by quantitative ELISA assay. Cytokine levels were expressed as mean ± SD in pg/mL. Paired samples -test was used to examine the differences. compared with LPS of IL-10.
431232.fig.001a
(a) TNF-α levels of monocytes in each group at different time points
431232.fig.001b
(b) IL-10 levels of monocytes in each group at different time points