Figure 2: Down-regulation of ADAM10 expression inhibits invasiveness of HepG2 cells. The HepG2 cells were transfected with medium only, the empty vector, the control siRNA, or siRNA against ADAM10. The Transwell invasiveness assay was performed. (a) The effect of ADAM10 knock-down on the invasiveness of HepG2 cells. (b) Diagram of the effect of ADAM10 knock-down on the invasiveness of HepG2 cells. A volume of 50 μL of the diluted Matrigel was added to the Transwell bottom chamber. A volume of 50 μL of DMEM was also added to the chamber. The cells that have migrated through the pores to the lower surface of the membrane were counted under microscope. Invasion ratios of HepG2 cells were calculated relative to the medium control. * when compared with the medium control.