434561.fig.003a
(a)
434561.fig.003b
(b)
Figure 3: Down-regulation of ADAM10 expression results in increased level of E-cadherin protein. HepG2 cells were transfected with medium only, the empty vector, the control siRNA, or siRNA against ADAM10. The total proteins were prepared and immunoblot analysis was performed to analyze the expression of E-cadherin, ADAM10, and β-actin. The cellular β-actin served as a loading control. (a) Representative blots were shown. (b) The relative E-cadherin protein levels were given. The Experiments were repeated for more than 3 times. * when compared with the level of E-cadherin in the medium control. # when compared with the level of ADAM10 in the medium control.