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BioMed Research International
Volume 2013 (2013), Article ID 457656, 7 pages
http://dx.doi.org/10.1155/2013/457656
Research Article

The Fc Receptor Polymorphisms and Expression of Neutrophil Activation Markers in Patients with Sickle Cell Disease from Western India

1Department of Hematogenetics, National Institute of Immunohaematology (Indian Council of Medical Research), 13th floor, New Multistoried Building, KEM Hospital Campus, Parel, Mumbai 400012, India
2Haematology Department, KEM Hospital, Parel, Mumbai 400012, India
3Valsad Raktadan Kendra, Valsad 396001, India
4Department of Pediatrics, Government Medical College, Nagpur 440003, India

Received 30 April 2013; Revised 2 August 2013; Accepted 27 August 2013

Academic Editor: Partow Kebriaei

Copyright © 2013 Harshada K. Kangne et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective. Sickle cell disease has variable clinical manifestations. Activation of neutrophils plays an important role in the initiation and propagation of vaso occlusive crises which can be analysed by determining the expression of neutrophil antigens such as CD16, CD32, and CD62L. The common FcγR polymorphisms (FcγRIIA and FcγRIIIB) are considered to influence clinical presentation. This study focuses on distribution of FcγR polymorphisms and their association with neutrophil activity among the patients from western India. Methods. In this paper 127 sickle cell anemia patients and 58 patients with sickle-β-thalassemia (median age  years) with variable clinical phenotypes along with 175 normals were investigated. FcγRs polymorphisms were analysed by RFLP and AS-PCR. Activation of neutrophils was measured by flow cytometry. Results. The genotypic frequency of the H/R genotype of FcγRIIA and the NA1/NA1 genotype of FcγRIIIB was significantly decreased in patients compared to normals ( -0.0074, -0.0471, resp.). We found a significant difference in the expression of CD32 and CD62L among the patients as against normals. A significantly higher expression of CD32 was seen in the milder patients with the H/H genotype ( -0.0231), whereas the expression of CD16 was higher in severe patients with the NA2/NA2 genotype ( -0.0312). Conclusion. The two FcγR polymorphisms had significant association with variable phenotypes of sickle cell disease. The expression of CD62L decreased in our patients indicating activation of neutrophils.