458571.fig.002a
(a)
458571.fig.002b
(b)
Figure 2: Dependence of values determined from stopped-flow experiments on pH, for T254A and T254C rcSHMT mutants. Open circles correspond to data obtained with L-serine, while closed circles correspond to data obtained with glycine. The continuous lines through the experimental points were obtained through a least square minimization process using the equation for a sigmoidal curve (the software used was GraphPad, Prism).