Structure-Based Mechanism for Early PLP-Mediated Steps of Rabbit Cytosolic Serine Hydroxymethyltransferase Reaction
Figure 2
Dependence of values determined from stopped-flow experiments on pH, for T254A and T254C rcSHMT mutants. Open circles correspond to data obtained with L-serine, while closed circles correspond to data obtained with glycine. The continuous lines through the experimental points were obtained through a least square minimization process using the equation for a sigmoidal curve (the software used was GraphPad, Prism).
