Table 1: Kinetic constants for the hydroxymethyltransferase and retro-aldol cleavage reactions catalyzed by wild type and T254 mutant rcSHMT forms.

SubstrateKinetic constantForm of rabbit cytosolic SHMT
Wild type T253AT253C

L-Serine (mM)0.30.60.5
(min−1)85018.51.6

L-allo-Threonine    (mM)1.52.52.6
(min−1)13014.34.5