Table 3: Crystallographic data collection and refinement statistics for rcSHMT structures determined in this work.

Wild typeT254AT254A + gly.T254A + L-ser.T254CT254C + gly.T254C + L-ser.

Cell/space group115.6,115.6,156.0
P41
115.1,115.1,157.4
P41
115.0,115.0,156.8
P41
115.5,115.5,156.3
P41
114.0,114.0,154.7
P41
114.6,114.6,156.9
P41212
114.0,114.0,155.6
P41
Resolution119.0–2.10 (2.16–2.10)111.8–2.55 (2.62–2.55) 20.0–2.65 (2.72–2.65)115–2.40 (2.46–2.40)111.8–2.40 (2.46–2.40)90.0–2.65 (2.72–2.65)111.8–2.55 (2.62–2.55)
Completeness (%)98.5 (98.2)92.2 (87.9)99.6 (99.8)88.3 (85.8)90.1 (86.9)69.6 (62.0)90.9 (76.9)
.199 (.248).201 (.306).203 (.292).197 (.247).195 (.242).221 (.333).202 (.284)
.242 (.316).271 (.432).277 (.399).258 (.318).256 (.346).305 (.414).263 (.362)
105631 (7668)55295 (3849)52905 (3769)63363 (4478)62364 (4393)19976 (1244)52787 (5963)
11736 (845)6204 (426)5968 (406)7156 (510)7009 (470)1654 (96)5963 (375)
30.844.83437.339.436.638
39.843.834.637.341.9NA40.1
RMSD from ideal
Bond lengths0.0080.0070.0070.0060.0060.0060.007
Bond angles1.31.241.221.131.131.081.16
Ramachandran plot
Favored146014241403142514357021427
Additionally allowed12314214214613369128
Generously allowed13891215612
Forbidden37955710
MolProbity score1.73 (93%)2.04 (96%)2.04 (97%)1.88 (96%)1.81 (97%)2.24 (93%)2.00 (96%)