Research Article
Structure-Based Mechanism for Early PLP-Mediated Steps of Rabbit Cytosolic Serine Hydroxymethyltransferase Reaction
Table 4
Rabbit cytosolic SHMT structures compared in this study. The table shows the cofactor form and the ligand present in each subunit of the structures.
| | Enzyme | Chain | Form | Ligand |
| | Unliganded wild type | A | i.a. | | | B | i.a. | | | C | i.a. | MES | | D | i.a. | MES |
| Unliganded
T254A | A | i.a. | | | B | i.a. | | | C | i.a. | | | D | i.a. | |
| | T254A + glycine | A | g.d. | Gly. | | B | g.d. | Gly. | | C | g.d. | Gly. | | D | g.d. | Gly. |
| | T254A + L-serine | A | g.d. | L-Ser. | | B | g.d. | L-Ser. | | C | g.d. | L-Ser. | | D | g.d. | L-Ser. |
| Unliganded
T254C | A | i.a. | H2O | | B | i.a. | H2O | | C | i.a. | H2O | | D | i.a. | PO4 |
| | T254C + glycine | A | g.d. | Gly. | | B | g.d. | Gly. |
| | T254C + L-serine | A | g.d. | L-Ser. | | B | g.d. | L-Ser. | | C | g.d. | L-Ser. | | D | g.d. | L-Ser. |
| | Wild type + glycine + 5-CHO-H4PteGlu3 | A | g.d. | Gly. | | B | i.a. | — | | C | g.d. | Gly. | | D | i.a. | — |
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i.a.: internal aldimine.
g.d.: gem-diamine.
MES: 2-(N-morpholino)ethanesulfonate.
5-CHO-H4PteGlu3: triglutamic form of 5-formyltetrahydrofolate.
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