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BioMed Research International
Volume 2013 (2013), Article ID 459196, 8 pages
http://dx.doi.org/10.1155/2013/459196
Research Article

Vasculoprotective Effects of Combined Endothelial Progenitor Cells and Mesenchymal Stem Cells in Diabetic Wound Care: Their Potential Role in Decreasing Wound-Oxidative Stress

1Inter-Department of Physiology, Graduate School, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
2Stem Cell and Cell Therapy Research Unit, Department of Pharmacology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
3King Chulalongkorn Memorial Hospital, Bangkok 10330, Thailand
4Department of Physiology, Center of Excellence for Microcirculation, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand

Received 9 March 2013; Revised 10 May 2013; Accepted 15 May 2013

Academic Editor: Dimitrios P. Bogdanos

Copyright © 2013 Supakanda Sukpat et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

To investigate whether the combined endothelial progenitor cells (EPCs) and mesenchymal stem cells (MSCs) could enhance angiogenesis and wound healing in diabetic mice. Balb/c nude mice were divided into five groups, including a control group, diabetic group (DM), DM injected with 1 × 106  cells MSCs, DM injected with 1 × 106  cells EPCs, and DM injected with combined 0.5 × 106  cells MSCs and 0.5 × 106  cells EPCs. After seven weeks, the mice were anesthetized, and bilateral full-thickness excision skin wounds were made on the dorsorostral back. The percentage of wound closure in DM group decreased significantly than in control and all other treated groups on day 7 and day 14 ( ). On day 14, the percentage of capillary vascularity in combine-treated group was significantly higher than in DM ( ). In the present study, we have demonstrated that the combined EPCs and MSCs can increase vascular endothelial growth factor (VEGF) level and angiogenesis which resulted in reduced neutrophil infiltration, decreased malondialdehyde (MDA) levels, and enhanced wound healing in diabetic mice model.