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BioMed Research International
Volume 2013 (2013), Article ID 460927, 7 pages
http://dx.doi.org/10.1155/2013/460927
Research Article

Lactobacillus plantarum LG42 Isolated from Gajami Sik-Hae Inhibits Adipogenesis in 3T3-L1 Adipocyte

1Department of Food Science and Human Nutrition, Chonbuk National University, Jeonju 561-756, Republic of Korea
2Jeonju Makgeolli Research Center, Chonbuk National University, Jeonju 561-756, Republic of Korea
3Department of Food and Biotechnology, Woosuk University, Jeonbuk 565-701, Republic of Korea

Received 3 October 2012; Accepted 17 December 2012

Academic Editor: Fabio Ferreira Perazzo

Copyright © 2013 Jeong-Eun Park et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We investigated whether lactic acid bacteria isolated from gajami sik-hae (GLAB) are capable of reducing the intracellular lipid accumulation by downregulating the expression of adipogenesis-related genes in differentiated 3T3-L1 cells. The GLAB, Lactobacillus plantarum LG42, significantly decreased the intracellular triglyceride storage and the glycerol-3-phosphate dehydrogenase (GPDH) activity in a dose-dependent manner. mRNA expression of transcription factors like peroxisome proliferator-activated receptor (PPAR) γ and CCAAT/enhancer-binding protein (C/EBP) α involved in adipogenesis was markedly decreased by the GLAB treatment. Moreover, the GLAB also decreased the expression level of adipogenic markers like adipocyte fatty acid binding protein (aP2), leptin, GPDH, and fatty acid translocase (CD36) significantly. These results suggest that the GLAB inhibits lipid accumulation in the differentiated adipocyte through downregulating the expression of adipogenic transcription factors and other specific genes involved in lipid metabolism.