Improvement of L-Arabinose Fermentation by Modifying the Metabolic Pathway and Transport in Saccharomyces cerevisiae

Table 1

S. cerevisiae strains and plasmids used in this study.

	Relevant genotype	Source/reference
Strain
CEN.PK102-3A	MATα leu2-3,  112 ura3-52	[17]
BSW1A1	CEN.PK102-3A derivative; {YIp5-ara}	This work
BSW1AY	CEN.PK102-3A derivative; {YIp5-ara, pYX242}	This work
BSW1A7	CEN.PK102-3A derivative; {YIp5-ara, pYX2422-HXT7araA}	This work
BSW1AT	CEN.PK102-3A derivative; {YIp5-ara, pYX2422-TEF1araA}	This work
BSW2AP	BSW1AT, gre3  (−241,  +338):: TPI1p-RKI1-RKI1t-PGK1p-TAL1-TAL1t-FBA1p-TKL1-TKL1t-ADH1p-RPE1-RPE1t-loxP	This work
BSW3AP	BSW2AP, selected for oxygen-limited growth on L-arabinose	This work
BSW3AG	BSW3AP derivative; {pJFE318-GAL2}	This work
Plasmid
pUG6	E. coli plasmid with segment LoxP-KanMX4-LoxP	[18]
pJPPP3	pUC19-based yeast integration plasmid, containing GRE3-targeting recombinant arms, overexpression cassette of Sc-TAL1, Sc-TKL1, Sc-RPE1, Sc-RKI1, and selectable marker loxP-KanMX4-loxP	[19]
YEp24-PGKp	2μ URA3	[20]
pHX	YEp24-PGKp PGK1p::HXT7p	This work
YIp5	Integration plasmid, Ura3	[21]
YIp5-ara	YIp5-HXT7p-araA-PGK1t-HXT7p-araB-PGK1t-HXT7p-araD-PGK1t, and selectable marker loxP-KanMX4-loxP	This work
pYX242	2μ LEU2	[22]
pYX242-WS	pYX242-PGK1t-TEF1p	This work
pYX2422-TEF1araA	pYX242-PGK1t-TEF1p-araA	This work
pYX2422-HXT7araA	pYX242-PGK1t-HXT7p-araA	This work
pJFE3	2μ URA3	[23]
pJFE3-GAL2	pJFE3-TEF1p-GAL2-PGK1t	This work
pJFE318-GAL2	pJFE3-GAL2 URA3::KanMX4	This work