461329.fig.003a
(a)
461329.fig.003b
(b)
461329.fig.003c
(c)
461329.fig.003d
(d)
Figure 3: Scanning electron microscopy of the interaction of A. polyphaga with MDCK cells. (a) No morphological evidence of damage was observed on control MDCK monolayers incubated for 3 h with 1 : 1 PBSGM : DMEM medium. Bar = 200 μm. (b) After 1 h of coincubation, small number of trophozoites were observed adhered to MDCK cells surface Bar = 20 μm. (c) By 2 h only few areas of monolayer discontinuity were observed, and a small number of trophozoites were located under and over the surface of MDCK cells. Bar = 10 μm. (d) At 3 h of coincubation, scarce areas devoid of cells were observed, in which a trophozoite was seen phagocyting fragments of MDCK cells. Bar = 10 μm.