Reevaluating the Role of Acanthamoeba Proteases in Tissue Invasion: Observation of Cytopathogenic Mechanisms on MDCK Cell Monolayers and Hamster Corneal Cells

Table 2

Summary of proteolytic activities detected through SDS-PAGE gels copolymerized with gelatin in total extracts (TE) and conditioned medium (CM) from of A. castellanii and A. polyphaga. The majority of proteases were inhibited 100% with PMSF (1 mM), only two of them were partially inhibited (*).

	Proteases; (kDa)	100% inhibition of proteases; PMSF (1 mM)
	TE, CM 120	CM 39*
	CM 97
	TE, CM 66
	TE, CM 64
	CM 57
A. castellanii	CM 55
	CM 50
	CM 47
	CM 45
	CM 40
	CM 38
	TE, CM 120
	CM 97
	CM 66
	CM 64
A. polyphaga	CM 57
	TE 55	TE 58*
	CM 50
	CM 40
	CM 38