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BioMed Research International
Volume 2013 (2013), Article ID 465179, 10 pages
http://dx.doi.org/10.1155/2013/465179
Research Article

Molecular Characterization and Clinical Impact of TMPRSS2-ERG Rearrangement on Prostate Cancer: Comparison between FISH and RT-PCR

1Laboratory of Molecular Biology, Fundación Instituto Valenciano de Oncología, C/Professor Beltrán Báguena 8, 46009 Valencia, Spain
2Department of Pathology, Fundación Instituto Valenciano de Oncología, C/Professor Beltrán Báguena 8, 46009 Valencia, Spain
3Department of Urology, Fundación Instituto Valenciano de Oncología, C/Professor Beltrán Báguena 8, 46009 Valencia, Spain
4Laboratori de Citogenètica Molecular, Servei de Patologia, GRETNHE, Programa de Recerca en Càncer, IMIM, Institut de Recerca del Hospital del Mar, Parc de Salut Mar, 08003 Barcelona, Spain
5Department of Pathology, Universitat de Valencia Estudi General, 46010 Valencia, Spain

Received 26 December 2012; Accepted 30 April 2013

Academic Editor: Sachidanand Pandey

Copyright © 2013 A. Fernández-Serra et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Prostate cancer (PCa) is a very heterogeneous disease, and there are constraints in its current diagnosis. Serum PSA levels, digital rectal examination (DRE), and histopathologic analysis often drive to overdiagnosis and overtreatment. Since 2005, the presence of the genetic rearrangement between transmembrane-serine protease gene (TMPRSS2) and the erythroblast transformation-specific (ETS) member ERG (v-ets erythroblastosis virus E26 oncogene homolog avian) has been demonstrated in almost half of PCa cases. Both FISH and RT-PCR are useful tools for detecting these rearrangements, but very few comparatives between both techniques have been published. In this study, we included FFPE tumors from 294 PCa patients treated with radical prostatectomy with more than 5 years of followup. We constructed a total of 20 tissue microarrays in order to perform break-apart and tricolor probe FISH approaches that were compared with RT-PCR, showing a concordance of 80.6% ( ). The presence of TMPRSS2-ERG rearrangement was observed in 56.6% of cases. No association between TMPRSS2-ERG status and clinicopathological parameters nor biochemical progression and clinical progression free survival was found. In conclusion, this study demonstrates that both FISH and RT-PCR are useful tools in the assessment of the TMPRSS2-ERG fusion gene status in PCa patients and that this genetic feature per se lacks prognostic value.