Preparation, Physicochemical Characterization, and Cell Viability Evaluation of Long-Circulating and pH-Sensitive Liposomes Containing Ursolic Acid

Table 1

Chemical and physicochemical properties of liposomes containing or not UA^a.

Formulation	Mean diameter (nm)	Zeta potential (mV)	UA entrapment (mg/mL)
SpHL 20B^b	105.8±3.3	3.2±1.0	—
SpHL 20UA1^c,h	191.1±6.4	1.2±1.4	0.77±0.01
SpHL 20UA0.5^d,h	149.4±1.5	1.7±2.6	0.38±0.01
SpHL 40B^e	107.3±2.6	2.9±0.4	—
SpHL 40UA1^f,h	140.4±1.2	4.1±1.3	0.78±0.05
SpHL 40UA0.5^g,h	122.8±3.1	4.4±0.5	0.35±0.04

aValues are expressed as mean ± S.D. (𝑛=3). ^bLipid concentration is equal to 20 mM, without UA. ^cLipid concentration is equal to 20 mM, and UA concentration is of 1 mg/mL. ^dLipid concentration is equal to 20 mM, and UA concentration is of 0.5 mg/mL. ^eLipid concentration is equal to 40 mM, without UA. ^fLipid concentration is equal to 40 mM, and UA concentration is of 1 mg/mL. ^gLipid concentration is equal to 40 mM, and UA concentration is of 0.5 mg/mL. ^hThere is a significant difference among the mean diameter values of SpHL samples containing UA and the respective SpHL samples without UA at the same lipid concentration (P < 0.05).