Stat3 Inhibits PTPN13 Expression in Squamous Cell Lung Carcinoma through Recruitment of HDAC5
Figure 4
Stat3 inhibits PTPN13 expression through being bound to its promoter region. (a) The PTPN13 promoter constructs containing a potential Stat3 binding motif (−626 to −618) (Wild type: Wt-Luc). Point mutations underlined were induced in the Stat3 motif (Mutant: Mut-Luc). (b) SK-MES-1 cells were cotransfected with the indicated plasmids for 36 h, and the luciferase activity was measured. (c) Two antibodies (antiIgG and -Stat3) were used in the ChIP assays using SK-MES-1 cells. Cells were incubated with vehicle control (Ctrl) or IL-6 (20 ng/mL) for 2 hr. Cells were then subjected to ChIP analysis and quantified by real-time PCR.
