Stat3 Inhibits PTPN13 Expression in Squamous Cell Lung Carcinoma through Recruitment of HDAC5
Figure 5
Stat3 represses PTPN13 expression through the recruitment of HDAC5. (a) Antibodies (antiIgG and -HDAC1, -HDAC2, -HDAC3, and -HDAC5) were used in the ChIP assays in SK-MES-1 cells. Cells were incubated with vehicle control (Ctrl) or IL-6 (20 ng/mL) for 2 hr. Cells were then subjected to ChIP analysis and quantified by real-time PCR. ((b)-(c)) mRNA and protein levels of PTPN13 were analyzed by Real-time PCR and western blot in SK-MES-1 cells transfected with empty vector (EV) or HDAC5. ((d)-(e)) SK-MES-1 cells were transfected for nonspecific control (Ctrl) or HDAC5 siRNA. Endogenous mRNA and protein levels of HDAC5 and PTPN13 were measured. ((f)-(g)) PTPN13 expression was analyzed by real-time PCR and western blot in SK-MES-1 cells treated with vehicle control (Ctrl) or IL-6 (20 ng/mL). Cells were pretransfected with nonspecific control (Ctrl) or HDAC5 siRNA for 24 hr.
