Effects of an Agaricus blazei Aqueous Extract Pretreatment on Paracetamol-Induced Brain and Liver Injury in Rats

Table 5

Effects of paracetamol injury and A. blazei pretreatment on metabolic parameters of perfused rat livers metabolizing exogenously supplied alanine. Livers from fasted rats were perfused according to the experimental protocol illustrated by Figure 2. For additional experimental details and see Section 2.

Metabolic fluxes (µmol min^−1 g^−1)	I, control	II, A. blazei pretreatment	III, paracetamol injury	IV, A. blazei pretreatment + paracetamol injury
Lactate production	0.31 ± 0.03 (𝑛=5)	0.25 ± 0.03 (𝑛=4)	0.62 ± 0.05 (𝑛=5)^a,b,c	0.35 ± 0.02 (𝑛=3)
Pyruvate production	0.13 ± 0.02 (𝑛=5)	0.082 ± 0.030 (𝑛=4)	0.29 ± 0.05 (𝑛=5)^a,b	0.20 ± 0.02 (𝑛=3)
Glucose production	0.25 ± 0.02 (𝑛=5)	0.21 ± 0.02 (𝑛=4)	0.092 ± 0.020 (𝑛=5)^a,b	0.060 ± 0.030 (𝑛=3)^d,e
Oxygen consumption	0.39 ± 0.06 (𝑛=5)	0.23 ± 0.05 (𝑛=4)	0.32 ± 0.07 (𝑛=5)	0.29 ± 0.03 (𝑛=3)
Ammonia production	0.058 ± 0.010 (𝑛=5)	0.085 ± 0.030 (𝑛=4)	0.20 ± 0.03 (𝑛=5)^a,b	0.16 ± 0.01 (𝑛=3)^d
Urea production	0.28 ± 0.01 (𝑛=5)	0.12 ± 0.01 (𝑛=4)^f,g	0.23 ± 0.03 (𝑛=5)^b	0.25 ± 0.05 (𝑛=3)

Significant differences (𝑃≤0.05), according to one-way ANOVA followed by Student-Newman-Keuls post-hoc testing, are identified by the superscript letters as follows: ^aIII versus I; ^bIII versus II; ^cIII versus IV; ^dIV versus I; ^eIV versus II; ^fII versus I; ^gII versus IV.