HGF Accelerates Wound Healing by Promoting the Dedifferentiation of Epidermal Cells through -Integrin/ILK Pathway
Figure 1
The gross and histological observation. (a) Photographs of full-thickness excisional punch wounds created in the skin of DB rats using a 2 cm biopsy tool 14 d prior to photography. After 14 d, the PUDKH group rat wounds are almost healed, while it took 20 days for wounds on rats in PBS and PUDK groups to heal. (b) Photographs of wounds were captured on 1, 3, 5, 7, 10, and 14 days after wounding to determine the degree of wound closure in DB rats. Graph represents the percentage of wound area at different times after wounding. The value means that there are differences between PUDKH group versus the PBS and PUDK groups animals at matched time point . (c) Hematoxylin/eosin staining showed a regularly stratified epithelium with ordinary developed hair follicles in normal rats. Reepithelialization of wounds in HGF gene-transfer rats was significantly increased on 5, 7, 10, and 14 days than that of the PBS and PUDK groups after gene transfer . On 14 d, regenerated epidermal cells layers recovered skin wounds of DB rats in PUDKH group; until 20 days, the rats in PBS and PUDK groups wound healed. 1: The epidermis of normal rat skin, 2: the sebaceous gland of normal rat skin, 3: the hair follicle of normal rat skin, 4: granuloma in rat skin wounds on 3 d, 5: reepithelialization in the wound edges on 7 d, 6: the regenerated epidermal cells layers recovered skin wounds on 14 d in PUDKH group, and 7: the newly-formed epithelium on 20 d.
