Figure 3: LCA induced apoptosis in T24 cells. The cells were treated with or without the indicated amounts of LCA for 48 h with or without NAC (500 μM). (a) Morphologic measurements in T24 cells were carried out via Hoechst fluorescence staining. (b) Detection of apoptotic rates conducted via flow cytometry. (c) Bcl-2 and Bax expressions were analyzed via RT-PCR (upper), and quantitative analysis of Bcl-2 and Bax expressions mRNA levels (lower). (d) Bcl-2 and Bax expressions were analyzed via western blot (upper), and quantitative analysis of Bcl-2 and Bax protein expressions (lower). Control group (LCA-untreated group) level was accepted to be “1.0”. , compared with the control group; , compared with the LCA group alone (50 μM).