Three-Dimensional Supermacroporous Carrageenan-Gelatin Cryogel Matrix for Tissue Engineering Applications
Figure 10
(a) Scanning electron micrographs of fibroblasts cells (Cos-7) in dividing stage on CGE cryogel matrix after 2nd day of culture at 5484x magnification, (b) Cos-7 on CGE after 7th day of culture at 1700x magnification, (c) Cos-7 cells on CGG after 2nd day of culture at 1861x magnification, and (d) Cos-7 on CGG after 7th day of culture with more ECM on matrix at 2865x magnification. (e), (f), (g) Fluorescence microscope images of fibroblast Cos-7 cells seeded on a 200 μm carrageenan-gelatin cryogel section analyzed by nuclear staining using PI stain. (e) PI staining image of cryogel with cells after 5th day of culture. (f) Composite image of cryogel with cells after 5th day of culture. (g) Surface plot of carrageenan-gelatin cryogel with cells.
