Epithelial Cell Culture from Human Adenoids: A Functional Study Model for Ciliated and Secretory Cells
Figure 6
Microphotograph of a confocal microscopy image of MUC5AC in cultures of secretory cells from adenoid epithelium. Cultures of secretory cells were fixed by means of 2% PFA and marked with two different antibodies against MUC5AC (green). (a) For the nonpermeabilized cells, antibody MAN5AC was used. (b) For the permeabilized cells, the polyclonal antibody MUC5AC (from Dr. Ho) was used. A diffuse label covering the cells surface was observed for the nonpermeabilized cells, as well as a discrete granular distribution for the permeabilized cells. Nuclei were stained with propidium iodide. Bar = 20 μm.
