- About this Journal
- Abstracting and Indexing
- Aims and Scope
- Annual Issues
- Article Processing Charges
- Articles in Press
- Author Guidelines
- Bibliographic Information
- Citations to this Journal
- Contact Information
- Editorial Board
- Editorial Workflow
- Free eTOC Alerts
- Publication Ethics
- Reviewers Acknowledgment
- Submit a Manuscript
- Subscription Information
- Table of Contents
BioMed Research International
Volume 2013 (2013), Article ID 478713, 13 pages
http://dx.doi.org/10.1155/2013/478713
Epithelial Cell Culture from Human Adenoids: A Functional Study Model for Ciliated and Secretory Cells
1Department of Otorhinolaryngology, Faculty of Medicine, Pontificia Universidad Católica de Chile, Marcoleta 350, 2°Piso, Santiago 8330033, Chile
2Department of Physiology, Faculty of Biological Sciences, Pontificia Universidad Católica de Chile, Alameda 340, Santiago 8331150, Chile
Received 9 August 2012; Revised 16 November 2012; Accepted 19 November 2012
Academic Editor: Anton M. Jetten
Copyright © 2012 Claudia González et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Linked References
- M. A. Sleigh, J. R. Blake, and N. Liron, “The propulsion of mucus by cilia,” American Review of Respiratory Disease, vol. 137, no. 3, pp. 726–741, 1988. View at Scopus
- N. A. Cohen, “Sinonasal mucociliary clearance in health and disease,” Annals of Otology, Rhinology, and Laryngology, vol. 196, pp. 20–26, 2006.
- L. Gheber, Z. Priel, C. Aflalo, and V. Shoshan-Barmatz, “Extracellular ATP binding proteins as potential receptors in mucociliary epithelium: characterization using [32P]3'-O-(4-benzoyl)benzoyl ATP, a photoaffinity label,” Journal of Membrane Biology, vol. 147, no. 1, pp. 83–93, 1995. View at Scopus
- T. Lieb, C. W. Frei, J. I. Frohock, R. J. Bookman, and M. Salathe, “Prolonged increase in ciliary beat frequency after short-term purinergic stimulation in human airway epithelial cells,” Journal of Physiology, vol. 538, no. 2, pp. 633–646, 2002. View at Publisher · View at Google Scholar · View at Scopus
- L. B. Wong and D. B. Yeates, “Luminal purinergic regulatory mechanisms of tracheal ciliary beat frequency,” American Journal of Respiratory Cell and Molecular Biology, vol. 7, no. 4, pp. 447–454, 1992. View at Scopus
- P. Roger, J. P. Gascard, J. Bara, V. T. De Montpreville, M. Yeadon, and C. Brink, “ATP induced MUC5AC release from human airways in vitro,” Mediators of Inflammation, vol. 9, no. 6, pp. 277–284, 2000. View at Publisher · View at Google Scholar · View at Scopus
- C. W. Davis, “Regulation of mucin secretion from in vitro celular models,” Novartis Foundation Symposium, vol. 248, pp. 113–131, 2002. View at Publisher · View at Google Scholar
- S. H. Donaldson, E. R. Lazarowski, M. Picher, M. R. Knowles, M. J. Stutts, and R. C. Boucher, “Basal nucleotide levels, release, and metabolism in normal and cystic fibrosis airways,” Molecular Medicine, vol. 6, no. 11, pp. 969–982, 2000. View at Scopus
- S. H. Donaldson, M. Picher, and R. C. Boucher, “Secreted and cell-associated adenylate kinase and nucleoside diphosphokinase contribute to extracellular nucleotide metabolism on human airway surfaces,” American Journal of Respiratory Cell and Molecular Biology, vol. 26, no. 2, pp. 209–215, 2002. View at Scopus
- E. R. Lazarowski, R. C. Boucher, and T. K. Harden, “Constitutive release of ATP and evidence for major contribution of ecto-nucleotide pyrophosphatase and nucleoside diphosphokinase to extracellular nucleotide concentrations,” Journal of Biological Chemistry, vol. 275, no. 40, pp. 31061–31068, 2000. View at Scopus
- T. Hayashi, M. Kawakami, S. Sasaki et al., “ATP regulation of ciliary beat frequency in rat tracheal and distal airway epithelium,” Experimental Physiology, vol. 90, no. 4, pp. 535–544, 2005. View at Publisher · View at Google Scholar · View at Scopus
- B. Morales, N. Barrera, P. Uribe, C. Mora, and M. Villalón, “Functional cross talk after activation of P2 and P1 receptors in oviductal ciliated cells,” American Journal of Physiology-Cell Physiology, vol. 279, no. 3, pp. C658–C669, 2000. View at Scopus
- B. Van Der Baan, “Ciliary function,” Acta Oto-Rhino-Laryngologica Belgica, vol. 54, no. 3, pp. 293–298, 2000. View at Scopus
- H. W. Hovenberg, J. R. Davies, A. Herrmann, C. J. Lindén, and I. Carlstedt, “MUC5AC, but not MUC2, is a prominent mucin in respiratory secretions,” Glycoconjugate Journal, vol. 13, no. 5, pp. 839–847, 1996. View at Publisher · View at Google Scholar · View at Scopus
- D. J. Thornton and J. K. Sheehan, “From mucins to mucus: toward a more coherent understanding of this essential barrier,” Proceedings of the American Thoracic Society, vol. 1, no. 1, pp. 54–61, 2004. View at Scopus
- C. Wickstrom, J. R. Davies, G. V. Eriksen, E. C. I. Veerman, and I. Carlstedt, “MUC5B is a major gel-forming, oligomeric mucin from human salivary gland, respiratory tract and endocervix: identification of glycoforms and C-terminal cleavage,” Biochemical Journal, vol. 334, no. 3, pp. 685–693, 1998. View at Scopus
- C. S. Rhee, Y. G. Min, C. H. Lee et al., “Ciliary beat frequency in cultured human nasal epitelial cells,” Annals of Otology, Rhinology, and Laryngology, vol. 110, no. 11, pp. 1011–1016, 2001.
- M. B. Antunes, B. A. Woodworth, G. Bhargave et al., “Murine nasal septa for respiratory epithelial air-liquid interface cultures,” BioTechniques, vol. 43, no. 2, pp. 195–204, 2007. View at Publisher · View at Google Scholar · View at Scopus
- T. E. Gray, K. Guzman, C. W. Davis, L. H. Abdullah, and P. Nettesheim, “Mucociliary differentiation of serially passaged normal human tracheobronchial epithelial cells,” American Journal of Respiratory Cell and Molecular Biology, vol. 14, no. 1, pp. 104–112, 1996. View at Scopus
- P. S. Mason, E. Adam, M. Prior, J. O. Warner, and C. J. Randall, “Effect of bacterial endotoxin and middle ear effusion on ciliary activity: implications for otitis media,” Laryngoscope, vol. 112, no. 4, pp. 676–680, 2002. View at Scopus
- B. Yang, R. J. Schlosser, and T. V. McCaffrey, “Dual signal transduction mechanisms modulate ciliary beat frequency in upper airway epithelium,” American Journal of Physiology-Lung Cellular and Molecular Physiology, vol. 270, no. 5, pp. L745–L751, 1996. View at Scopus
- B. Yang, R. J. Schlosser, and T. V. McCaffrey, “Signal transduction pathways in modulation of ciliary beat frequency by methacholine,” Annals of Otology, Rhinology and Laryngology, vol. 106, no. 3, pp. 230–236, 1997. View at Scopus
- Z. Weisman and J. Sadé, “Tissue culture of human adult adenoids and of middle ear mucosa,” Annals of Otology, Rhinology, and Laryngology, vol. 85, part 1, no. 3, pp. 327–323, 1976.
- P. Verdugo, R. E. Rumery, and P. Y. Tam, “Hormonal control of oviductal ciliary activity: effect of prostaglandins,” Fertility and Sterility, vol. 33, no. 2, pp. 193–196, 1980. View at Scopus
- N. P. Barrera, B. Morales, and M. Villalón, “Plasma and intracellular membrane inositol 1,4,5-trisphosphate receptors mediate the Ca2+ increase associated with the ATP-induced increase in ciliary beat frequency,” American Journal of Physiology-Cell Physiology, vol. 287, no. 4, pp. C1114–C1124, 2004. View at Publisher · View at Google Scholar · View at Scopus
- M. Villalon, T. R. Hinds, and P. Verdugo, “Stimulus-response coupling in mammalian ciliated cells. Demonstration of two mechanisms of control for cytosolic [Ca2+],” Biophysical Journal, vol. 56, no. 6, pp. 1255–1258, 1989. View at Scopus
- N. P. Barrera, B. Morales, and M. Villalon, “ATP and adenosine trigger the interaction of plasma membrane IP3 receptors with protein kinase A in oviductal ciliated cells,” Biochemical and Biophysical Research Communications, vol. 364, no. 4, pp. 815–821, 2007. View at Publisher · View at Google Scholar · View at Scopus
- R. Wu, Y. H. Zhao, and M. M. Chang, “Growth and differentiation of conducting airway epithelial cells in culture,” European Respiratory Journal, vol. 10, no. 10, pp. 2398–2403, 1997. View at Publisher · View at Google Scholar
- J. V. Small and J. E. Celis, “Filament arrangements in negatively stained cultured cells: the organization of actin,” Cytobiologie, vol. 16, no. 2, pp. 308–325, 1978. View at Scopus
- S. B. Ho, K. Takamura, R. Anway, L. L. Shekels, N. W. Toribara, and H. Ota, “The adherent gastric mucous layer is composed of alternating layers of MUC5AC and MUC6 mucin proteins,” Digestive Diseases and Sciences, vol. 49, no. 10, pp. 1598–1606, 2004. View at Publisher · View at Google Scholar · View at Scopus
- K. Rousseau, C. Wickstrom, D. B. Whitehouse, I. Carlstedt, and D. M. Swallow, “New monoclonal antibodies to non-glycosylated domains of the secreted mucins MUC5B and MUC7,” Hybridoma and Hybridomics, vol. 22, no. 5, pp. 293–299, 2003. View at Scopus
- J. K. Sheehan, S. Kirkham, M. Howard et al., “Identification of molecular intermediates in the assembly pathway of the MUC5AC mucin,” Journal of Biological Chemistry, vol. 279, no. 15, pp. 15698–15705, 2004. View at Publisher · View at Google Scholar · View at Scopus
- Y. Chen, Yu Hua Zhao, and R. Wu, “Differential regulation of airway mucin gene expression and mucin secretion by extracellular nucleotide triphosphates,” American Journal of Respiratory Cell and Molecular Biology, vol. 25, no. 4, pp. 409–417, 2001. View at Scopus
- M. Kesimer, C. Ehre, K. A. Burns, C. W. Davis, J. K. Sheehan, and R. J. Pickles, “Molecular organization of the mucins and glycocalyx underlying mucus transport over mucosal surfaces of the airways,” Mucosal Immunology. In press. View at Publisher · View at Google Scholar
- B. W. T. Yin and K. O. Lloyd, “Molecular cloning of the CA125 ovarian cancer antigen: identification as a new mucin, MUC16,” Journal of Biological Chemistry, vol. 276, no. 29, pp. 27371–27375, 2001. View at Publisher · View at Google Scholar · View at Scopus
- F. J. Rohlf and R. R. Sokal, Statistical Tables, W. H. Freeman and Company, New York, NY, USA, 1969.
- J. K. Sheehan, C. Brazeau, S. Kutay et al., “Physical characterization of the MUC5AC mucin: a highly oligomeric glycoprotein whether isolated from cell culture or in vivo from respiratory mucous secretions,” Biochemical Journal, vol. 347, no. 1, pp. 37–44, 2000. View at Publisher · View at Google Scholar · View at Scopus
- J. A. Voynow and B. K. Rubin, “Mucins, mucus, and sputum,” Chest, vol. 135, no. 2, pp. 505–512, 2009. View at Publisher · View at Google Scholar
- S. M. Gayner and T. V. McCaffrey, “Muscarinic ciliostimulation requires endogenous prostaglandin production,” American Journal of Rhinology, vol. 12, no. 3, pp. 203–207, 1998. View at Scopus
- P. J. Schuil, M. Ten Berge, J. M. E. Van Gelder, K. Graamans, and E. H. Huizing, “Substance P and ciliary beat of human upper respiratory cilia in vitro,” Annals of Otology, Rhinology and Laryngology, vol. 104, no. 10, pp. 798–802, 1995. View at Scopus
- P. A. Staskowski and T. V. McCaffrey, “Effect of substance P on ciliary beat frequency in human adenoid explants,” Otolaryngology-Head and Neck Surgery, vol. 107, no. 4, pp. 553–557, 1992. View at Scopus
- P. J. Schuil, K. Graamans, and E. E. Huizing, “Cell suspension cultures and adenoid epithelium: an assessment of the source of material for human ciliary function experiments in vitro,” Rhinology, vol. 33, no. 2, pp. 66–69, 1995. View at Scopus
- D. M. Morse, J. L. Smullen, and C. W. Davis, “Differential effects of UTP, ATP, and adenosine on ciliary activity of human nasal epithelial cells,” American Journal of Physiology-Cell Physiology, vol. 280, no. 6, pp. C1485–C1497, 2001. View at Scopus
- L. Zhang and M. J. Sanderson, “Oscillations in ciliary beat frequency and intracellular calcium concentration in rabbit tracheal epithelial cells induced by ATP,” Journal of Physiology, vol. 546, no. 3, pp. 733–749, 2003. View at Publisher · View at Google Scholar · View at Scopus
- J. K. Sheehan, M. Howard, P. S. Richardson, T. Longwill, and D. J. Thornton, “Physical characterization of a low-charge glycoform of the MUC5B mucin comprising the gel-phase of an asthmatic respiratory mucous plug,” Biochemical Journal, vol. 338, no. 2, pp. 507–513, 1999. View at Publisher · View at Google Scholar · View at Scopus
- M. Espinosa, G. Noé, C. Troncoso, S. B. Ho, and M. Villalón, “Acidic pH and increasing [Ca2+] reduce the swelling of mucins in primary cultures of human cervical cells,” Human Reproduction, vol. 17, no. 8, pp. 1964–1972, 2002. View at Scopus
- P. Verdugo, “Hydration kinetics of exocytosed mucins in cultured secretory cells of the rabbit trachea: a new model,” Ciba Foundation symposium, vol. 109, pp. 212–225, 1984. View at Scopus
- B. J. Van Klinken, A. W. C. Einerhand, H. A. Büller, and J. Dekker, “Strategic biochemical analysis of mucins,” Analytical Biochemistry, vol. 265, no. 1, pp. 103–116, 1998. View at Publisher · View at Google Scholar · View at Scopus
- P. Roger, J. P. Gascard, J. Bara, V. T. De Montpreville, M. Yeadon, and C. Brink, “ATP induced MUC5AC release from human airways in vitro,” Mediators of Inflammation, vol. 9, no. 6, pp. 277–284, 2000. View at Publisher · View at Google Scholar · View at Scopus
- S. Okada, L. Zhang, S. Kreda et al., “Coupled nucleotide and mucin hypersecretion from goblet-cell metaplastic human airway epithelium,” American Journal of Respiratory Cell and Molecular Biology, vol. 45, no. 2, pp. 253–260, 2011. View at Publisher · View at Google Scholar