Research Article

Genetic and Biochemical Diversity of Paenibacillus larvae Isolated from Tunisian Infected Honey Bee Broods

Figure 4

REP-PCR using BOX primer. (a) The relationship between the isolates of P. larvae and other Paenibacillus species detected on agarose gels: lane 1: P. macerans; lane 2: P. alvei A4; lane 3: P. thiaminolyticus; lane 4: P. alvei A3; lane 5: P. dendritiformis; lane 6: P. vorticalis; lane 7: P. polymyxa. A, B, and C: three BOX haplotypes detected on agarose gel. (b) BOX-PCR profile of P. larvae isolates detected on 6% polyacrylamide gels; six BOX haplotypes were detected for 75 isolates (G1 to G6). m: marker 50 bp; M: marker 100 bp; the additional bands detected on polyacrylamide gel were indicated with arrowheads.
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