480620.fig.003a
(a)
480620.fig.003b
(b)
Figure 3: PAS staining and immunohistochemistry. (a) PAS and immunohistochemistry staining patterns in the white portion of the medial gastrocnemius. (b) PAS and immunohistochemistry staining patterns in red portion of the medial gastrocnemius. In PAS staining, the intramuscular glycogen content is indicated by a deep purple color. In immunohistochemistry, myosin heavy chain I (black) and II (green) are indicated. IHC: immunohistochemistry. HFS: high-frequency electrical stimulation group. LFS: low-frequency electrical stimulation group. HFS T and LFS T: the trained leg in HFS and LFS, respectively.