480702.fig.005a
(a)
480702.fig.005b
(b)
Figure 5: (a, b) CX3CL1 expression in the SC of EAE12 rats. Double-labeled immunofluorescence for CX3CL1 and markers for neurons (NeuN) or astrocytes (GFAP) in the SC of EAE12 rats. (a) CX3CL1 was expressed in neurons in the grey matter of SC. CX3CL1 (panels i and iv: red) in neurons (NeuN: panels ii and v: green). CX3CL1 co-localizes with neurons (panels iii and vi: yellow, arrows). (b) Colocalization between CX3CL1 and astrocytes was observed in the grey matter of SC. CX3CL1 (panels i and iv: red) in astrocytes (GFAP: panels ii and v: green). CX3CL1 colocalizes with astrocytes (panels iii and vi: yellow, arrows). Images were taken at a total magnification of 100x (panels i–iii) and 400x (panels iv–vi) from EAE12 group. Scale bars = 10 μm.