480702.fig.006a
(a)
480702.fig.006b
(b)
Figure 6: (a, b) CX3CR1 expression in the SC of EAE12 rats. Double-labeled immunofluorescence for CX3CR1 and markers for microglia (CD68) or neurons (NeuN) in the SC of EAE12 rats. (a) Colocalization between CX3CR1 and neurons was observed in the dorsal horn (in asterisk) of SC. CX3CR1 (panels i and iv: red) in neurons (NeuN: panels ii and v: green). CX3CR1 colocalizes with neurons (panels iii and vi: yellow, arrows). (b) CX3CR1 was expressed in microglia in the grey matter of SC. CX3CR1 (panels i and iv: red) in microglia (CD68: panels ii and v: green). CX3CR1 colocalizes with microglia (panels iii and vi: yellow, arrows). Images were taken at a total magnification of 100x (panels i–iii) and 400x (panels iv–vi) from EAE12 group. Scale bars = 10 μm.