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BioMed Research International
Volume 2013 (2013), Article ID 489032, 8 pages
http://dx.doi.org/10.1155/2013/489032
Research Article

Does Cryopreservation of Ovarian Tissue Affect the Distribution and Function of Germinal Vesicle Oocytes Mitochondria?

1Anatomy Department, Tarbiat Modares University, P.O. Box 14115-111, Tehran, Iran
2Division of Medical Nutrition, Department of Biosciences and Nutrition, Karolinska University Hospital, Karolinska Institutet, Novum, 141 86 Stockholm, Sweden
3School of Biology, Damghan University, P.O. Box 3671641167, Damghan, Iran

Received 6 April 2013; Revised 16 June 2013; Accepted 17 June 2013

Academic Editor: Heide Schatten

Copyright © 2013 Mojdeh Salehnia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to evaluate mitochondrial alteration and ATP content of germinal vesicle (GV) oocytes isolated from fresh and vitrified ovaries. After superovulation, the ovaries from adult mice were collected and divided into control and vitrified groups. GV oocytes were isolated mechanically from each group. Half were cultured for 24 hours and their maturation was assessed. Metaphase II oocytes were collected and submitted to in vitro fertilization and their fertilization rates and development to the blastocyst stage were evaluated. In the remaining GV oocytes, ATP levels were quantified, and mitochondrial distribution, mitochondrial membrane potential, and intracellular free calcium were detected with rhodamine 123, JC-1 and Flou-4 AM staining, using laser-scanning confocal microscopy. Maturation and fertilization rates of GV oocytes and the developmental rates of subsequent embryos were significantly lower in vitrified samples ( ). The ATP content and Ca2+ levels differed significantly in fresh and vitrified GV oocytes ( ). Most mitochondria were seen as large and homogenous aggregates (66.6%) in fresh GV oocytes compared to vitrified oocytes (50%). No significant differences in mitochondrial membrane potential were found between the groups. The lower maturation and fertilization rates of GV oocytes from vitrified ovaries may be due to changes in their mitochondrial function and distribution.